Countercurrent Separation Tutorials & Workshop Materials
You will find in this webpage a series of workshop materials on countercurrent separation. These educative slides were prepared by Dr. Friesen and presented during the Chicago CCC2016 meeting. You can also click on the logo 
to access the chosen presentation.
Table of Contents
- Instrument Introduction
- Solvent System Selection
- Optimization Parameters
- Elution Methods
- Instrument Methods
- Reciprocal Symmetry Plots
- K-Based Chromatography
- Other links towards educative contents
1. Instrument Introduction ☞
Introduction to countercurrent chromatography: instruments from Center for Natural Product Technologies
Useful links related to this presentation and cited research articles:
- Countercurrent Extraction - Craig Apparatus
- HSCCC and natural food pigments
- The concept of resolution in chromatography
- Countercurrent Separation of Natural Products: An Update
- Purity of Crystalline Penicillins
- Droplet counter-current chromatography for the separation of plant products
- Binary concepts and standardization in counter-current separation technology
- Countercurrent Chromatography and the Journal of Liquid Chromatography: A Love Story
1a. Commercially Available Instruments ☞
Commercially Available Countercurrent Separation (CCS) Instruments from Center for Natural Product Technologies
Useful links related to this presentation:
1b. Instrument Comparison ☞
Cited research articles related to this presentation:
- Countercurrent Separation of Natural Products: An Update
- Development of Circulating Ultrasounic-Assisted Online Extraction Coupled to Countercurrent Chromatography and Centrifugal Partition Chromatography for Simultaneous Extraction and Isolation of Phytochemicals
- Advantages of a small-volume counter-current chromatography column
- Performance comparison using the GUESS mixture to evaluate counter-current chromatography instruments
2. Solvent System Selection
2a. Solvent System Formulation
2b. Partitioning Experiments
How to perform paritioning experiments to calculate the partition coefficent K of target compounds: A fundamental step for the selection of solvent systems.
2c. Phase Modifications
What are the different parameters to consider for the modification of a chosen solvent system to be utilized in countercurrent chromatography.
3. Optimization Parameters
Optimization parameters in Countercurrent Chromatography from Center for Natural Product Technologies
What are the different parameters to modify or control (e.g. sample loading, temperature of elution, rotation speed, flow rate) in order to optimize the separation of target compounds in countercurrent chromatography?
4. Elution Methods
Some details on the different elution methods (e.g., EECCC, BECCC, Dual mode, recycling mode) that can be implemented in countercurrent chromatography.
☞ Youtube 🎥 Elution Extrusion Countercurrent Chromatography (EECCC)
5. Instrument Methods
5a. Solvent Delivery Methods
Different solvent delivery methods in Counterurrent Chromatography from Center for Natural Product Technologies
Different types of solvent delivery methods from «on demand» solvent mixing to ion exchange with a particular focus on pH-zone refining
6. Reciprocal Symmetry Plots
Reciprocal symmetry plots in Countercurrent Chromatography from Center for Natural Product Technologies
How to represent Countercurrent chromatographic results and what are the Reciprocal Symmetry plots
7. K-Based Chromatography
K based-chromatography: future and potential of Countercurrent Chromatography from Center for Natural Product Technologies
Future and potential of Countercurrent Chromatography from preparative isolation of compounds to the production of Knock-out Extracts. How can CCC become a mainstream technique?