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Countercurrent Separation Tutorials & Workshop Materials

You will find in this webpage a series of workshop materials on countercurrent separation. These educative slides were prepared by Dr. Friesen and presented during the Chicago CCC2016 meeting. You can also click on the logo slideshare Logo to access the chosen presentation.

Table of Contents

  1. Instrument Introduction
  2. Solvent System Selection
  3. Optimization Parameters
  4. Elution Methods
  5. Instrument Methods
  6. Reciprocal Symmetry Plots
  7. K-Based Chromatography
  8. Other links towards educative contents

Introduction to countercurrent chromatography: instruments from Center for Natural Product Technologies

Commercially Available Countercurrent Separation (CCS) Instruments from Center for Natural Product Technologies

Comparison of CCS Instruments from Center for Natural Product Technologies

2. Solvent System Selection

Solvent System Selection from Center for Natural Product Technologies

2a. Solvent System Formulation

solvent system formulation from Center for Natural Product Technologies

2b. Partitioning Experiments

Partitioning experiments from Center for Natural Product Technologies
How to perform paritioning experiments to calculate the partition coefficent K of target compounds: A fundamental step for the selection of solvent systems.

2c. Phase Modifications

Phase modifications from Center for Natural Product Technologies
What are the different parameters to consider for the modification of a chosen solvent system to be utilized in countercurrent chromatography.

3. Optimization Parameters

Optimization parameters in Countercurrent Chromatography from Center for Natural Product Technologies
What are the different parameters to modify or control (e.g. sample loading, temperature of elution, rotation speed, flow rate) in order to optimize the separation of target compounds in countercurrent chromatography?

4. Elution Methods

Elution methods in Countercurrent Chromatography from Center for Natural Product Technologies
Some details on the different elution methods (e.g., EECCC, BECCC, Dual mode, recycling mode) that can be implemented in countercurrent chromatography.

☞ Youtube 🎥 Elution Extrusion Countercurrent Chromatography (EECCC)

5. Instrument Methods

Instrument Methods (Introduction) from Center for Natural Product Technologies

5a. Solvent Delivery Methods

Different solvent delivery methods in Counterurrent Chromatography from Center for Natural Product Technologies
Different types of solvent delivery methods from «on demand» solvent mixing to ion exchange with a particular focus on pH-zone refining

6. Reciprocal Symmetry Plots

Reciprocal symmetry plots in Countercurrent Chromatography from Center for Natural Product Technologies
How to represent Countercurrent chromatographic results and what are the Reciprocal Symmetry plots

7. K-Based Chromatography

K based-chromatography: future and potential of Countercurrent Chromatography from Center for Natural Product Technologies
Future and potential of Countercurrent Chromatography from preparative isolation of compounds to the production of Knock-out Extracts. How can CCC become a mainstream technique?